Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the pleural effusion of a 53-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1970; cells carry the BCR-ABL1 e14-a2 (b3-a2) fusion gene; this subclone (ACC 10) weakly expresses MHC class I (
image); other subclones (ACC 86 and ACC 92) are MHC class I negative and might be used in in-vitro natural killer assays; cells produce hemoglobin. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-1.0 x 106 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximal density at 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays
Immunology:
CD3 -, CD14 -, CD15 +, CD19 -, CD33 +, CD71 +, CD235a +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with sharp mode - 61-68<3n>XX, -X, -3, +7, -13, -18, +3mar, del(9)(p11/13), der(14)t(14;?)(p11;?), der(17)t(17;?)(p11/13;?), der(?18)t(15;?18)(q21;?q12), del(X)(p22) - two markers appear from FISH to have arisen from Ph;
fusion gene BCR-ABL1 is co-amplified together with MIR17HG (13q31)
Molec. Genetics:
expression of fusion gene BCR-ABL1 (e14-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
