CHO-K1
ACC 110
Cell line:
CHO-K1
DSMZ no.:
ACC 110
Species:
Chinese hamster (Cricetulus griseus)
Cell type:
ovary cells
Origin:
subclone from parental CHO cell line with distinctive karyotype; CHO cell line was initiated from an ovary biopsy of an adult Chinese hamster in 1957
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Ham's F12 + 10% h.i. FBS
Subculture:
seed out about 105 - 106 cells in an 80 cm2 flask; split confluent cultures 1:3 to 1:10 using trypsin/EDTA once or twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
about 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species.
Cytogenetics:
near diploid karyotype with 12% polyploidy - 22(20-23) - exhibits distinctive CHO karyology
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list