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DetailsCatalogue
Cell line:
SF-9
DSMZ no.:
ACC 125
Species:
insect - fall armyworm (Spodoptera frugiperda) (Spodoptera frugiperda)
Cell type:
ovary cells
Origin:
cloned from the parent cell line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors
Reference(s):
14967, 15043
Depositor:
Dr. Asta Cramer, Institute for Virology, Marburg, Germany
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
polymorphic round cells, mostly adherent, a few cells in suspension
Medium:
80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate)
Subculture:
split culture 1:2 to 1:3 every 3-5 days (cells grow adherent, but trypsin is not necessary as cells can be detached by tapping the flask or with a rubber policeman); seed out at ca. 2 x 106 cells/80 cm2; immediately after thawing, cell viability may be low
Incubation:
25-29 °C without CO2 (keep lid of flask closed); temperature below 23 °C may lead to reduced growth and loss of adherence
Doubling time:
ca. 50 hours
Harvest:
about 5-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species:
exclusion of primate and rodent origin by IEF and species PCR
Viruses:
PCR: SMRV -
Supplied as:
Delivery form Prices
Growing culture
  (please inquire for exact delivery time)
 660,- €
Frozen culture 330,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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