- Cell line:
- SF-9
- DSMZ no.:
- ACC 125
- Species:
- insect - fall armyworm (Spodoptera frugiperda) (Spodoptera frugiperda)
- Cell type:
- ovary cells
- Origin:
- cloned from the parent cell line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors
- Reference(s):
- 14967, 15043
- Depositor:
- Dr. Asta Cramer, Institute for Virology, Marburg, Germany
- Biosafety level:
- 1
- Permissions and restrictions:
- A
- DSMZ Cell Culture Data:
- Morphology:
- polymorphic round cells, mostly adherent, a few cells in suspension
- Medium:
- 80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate)
- Subculture:
- split culture 1:2 to 1:3 every 3-5 days (cells grow adherent, but trypsin is not necessary as cells can be detached by tapping the flask or with a rubber policeman); seed out at ca. 2 x 106 cells/80 cm2; immediately after thawing, cell viability may be low
- Incubation:
- 25-29 °C without CO2 (keep lid of flask closed); temperature below 23 °C may lead to reduced growth and loss of adherence
- Doubling time:
- ca. 50 hours
- Harvest:
- about 5-10 x 106 cells/80 cm2
- Storage:
- frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule
- DSMZ Scientific Data:
- Mycoplasma:
- negative in DAPI, microbiological culture, RNA hybridization, PCR assays
- Species:
- exclusion of primate and rodent origin by IEF and species-PCR
- Viruses:
- PCR: SMRV -
- Supplied as:
Delivery form Prices Frozen culture 300,- € Growing culture
(please inquire for exact delivery time)600,- € DNA isolated from cell line (25 µg) 400,- € Cell pellet (10-20 x 106 cells) 500,- €
see price list- Print data sheet
Stellenausschreibungen:
W2-Professur „Mikrobielle Genomforschung“
Wiss. Mitarbeiter/-in iDiv (Postdoc)
25. Mai Tag der offenen Tür
