SUP-T1
ACC 140
Cell line:
SUP-T1
DSMZ no.:
ACC 140
Species:
human (Homo sapiens)
Cell type:
T cell lymphoma
Origin:
established from the pleural effusion of an 8-year-old boy with T-lymphoblastic lymphoma in relapse
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 2-3 days; after thawing the viability may drop to less than 50%, but the cells recover during the following 4-6 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
maximal density of 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, thereafter negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
cyCD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 1.8% polyploidy - 85<4n>XXX/XXX?Y, -8, -8, -9, -12, inv(2)(p22q11)x2, t(2;?20)(p13;?p11), del(4)(q31q35), del(6)(q25)x2, add(7)(q32), add(9)(q34)x2, inv(14)(q11q32)x2 - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list