HUP-T4
ACC 223
Cell line:
HUP-T4
DSMZ no.:
ACC 223
Species:
human (Homo sapiens)
Cell type:
pancreas adenocarcinoma
Origin:
established from the malignant ascites of a 60-year-old man with pancreas carcinoma in 1985 (histologically as well differentiated papilotubular adenocarcinoma); described to be tumorgenic in nude mice and production of CEA and CA 19-9
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% MEM (with Earle's salts) + 20% h.i. FBS + 1% non-essential amino acids + 1% sodium pyruvate
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split 1:2 to 1:3 every 2-5 days using trypsin/EDTA (recommended: trypsin/EDTA for 10 min at 37 °C)
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 38 hours
Harvest:
cell harvest of about 1-2 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-triploid karyotype with 11% polyploidy and 1-2 supernumerary dm - 69/70(66-73)<3n>X, ?del(X)/XY, -Y, -2, -4, +6, +7, +8, -9, -9, +11, +16, +mar - inv(3)(p22q24), i(8q), idic(9)(pter), add(17)(p12) - 2 medium size ring ch present at 10%
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list