IPC-298
ACC 251
Cell line:
IPC-298
DSMZ no.:
ACC 251
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the primary tumor (right cervical) of a 64-year-old woman with cutaneous melanoma
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
seed out at ca. 2-3 x 106 cells/80 cm2;
split confluent culture 1:5 to 1:10 every 4-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30 hours
Harvest:
cell harvest of about 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 13% polyploidy - 74(74-88)<3n>XX, -X, +1, -2, +3, -4, +6, +7, +9, -11, -11, +12, -13, -14, -15, +16, +21, +21, +4mar, dmin in 34% cells, del(3)(p14)x2, del(3)(q26.3)x2, del(6)(p23), del(7)(p14), add(7)(p22;HSR), der(9)t(9;11)(p21;q11), add(12)(p13)x3, del(12)(p13), del(17)(p12)x1-2 - sidelines with additional numerical and structural ch 7 rearrangements
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the NRAS Q61L mutation (mu/wt), but no BRAF V600 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list