CADO-ES1
ACC 255
Cell line:
CADO-ES1
DSMZ no.:
ACC 255
Species:
human (Homo sapiens)
Cell type:
Ewing's sarcoma
Origin:
established from the malignant pleural effusion from a 19-year-old woman diagnosed with right-buttock Ewing's sarcoma with multiple lung metastases; described to be heterotransplantable, capable of anchorage-independent growth and able to neural differentiation in response to cAMP
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 2-3 x 106 cells in a 80 cm2 flask; split confluent culture 1:4 to 1:10 every 3-7 days using trypsin/EDTA; after thawing use 20% FBS at first
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of about 30 x 106 cells/80cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 8% polyploidy - 47(44-48)<2n>XX, +8, dup(1)(q32q44), i(8q), add(18)(p11.32) - t(11;22) absent - corresponds to published karyotype - absence of EWS-FLI1 fusion transcripts confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list