SW-948
ACC 326
Cell line:
SW-948
DSMZ no.:
ACC 326
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from the colon tumor of a 81-year-old woman with colon adenocarcinoma (Duke C)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Leibovitz's L-15 + 10% h.i. FBS
Subculture:
split confluent culture optimally at 1:2 to 1:7 once or twice a week using trypsin/EDTA; seed out at ca. 6 x 104 cells/cm2 or 2 x 106 cells/25 cm2 flask
Incubation:
at 37 °C without CO2 gas exchange (no filter lid, lid of flask closed)
Doubling time:
2-4 days
Harvest:
cell harvest of 2.0-2.5 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 12% polyploidy - 68(65-70)<3n>XXX, +7, -8, -10, +13, +13, -16, +2mar, del(X)(q22.2), del(1)(p12), i(9q), add(17)(p12), add(19)(q13)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list