HD-MY-Z
ACC 346
Cell line:
HD-MY-Z
DSMZ no.:
ACC 346
Species:
human (Homo sapiens)
Cell type:
Hodgkin lymphoma
Origin:
established in 1991 from the pleural effusion (rich in Hodgkin/Reed-Sternberg cells) from a 29-year-old patient with nodular sclerosing Hodgkin lymphoma (stage IIIb) refractory to therapy
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.2-0.75 x 106 cells/ml or 0.8-1.6 x 105 cells/cm2; optimal split ratio of 1:3 to 1:8 every 2-5 days; cells may be dislodged mechanically with trypsin/EDTA; seed out at ca. 0.3-0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
saturation density at ca. 1.5 x 106 cells/ml or 5 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD2 -, CD3 -, CD10 +, CD13 +, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 -, cyCD68 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human highly rearranged hyperdiploid karyotype with 24% polyploidy - 52-55<2n>X/Xdel(X)(p11), +1, +2, +5, +6, +8, +9, -17, -17, -18, +19, +20, +21, +22, +2mar, der(1)t(1;3)(q32;p21), der(2)t(2;?10)(p25;q22), add(3)(q27), del(4)(q22), i(5p), i(5q), der(5)t(5;8)(p14.1;p12), del(6)(q15), add(7)(p15), add(7)(p11), del(8)(q11), der(9)add(9)(p24)add(9)(q21), der(10)add(10)(p11)add(10)(q22), add(11)(q24), i(13q), der(13)t(7;13)(q11;p13), add(18)(p13), add(19)(q13.1) - additional rearrangements present in subclones include der(1)t(1;2)(q11;p25), del(3)(q21), add(16)(q24)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list