DBTRG-05MG
ACC 359
Cell line:
DBTRG-05MG
DSMZ no.:
ACC 359
Species:
human (Homo sapiens)
Cell type:
glioblastoma
Origin:
established from the primary tumor cells of a 59-year-old woman with glioblastoma multiforme following irradiation and chemotherapy in 1985; cells were described in the literature to express NSE (neuron-specific enolase) and EGF-R (epidermal growth factor receptor)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype with 12% polyploidy - 81-88<4n>XXX, -X, -1, -1, -3, -4, -4, +7, +7, -10, -10, -13, -13, -14, -14, -16, +6mar, der(4)t(4;14)(p11;q11), add(5)(p15)x2, add(7)(p22)x3, del(8)(q22), inv(9)(p13q34)x1-2, add(11)(p15)x2, der(11)t(1;11)(q23;q21), del(11)(q13), add(16)(q24)x2 - +7 and -10 recurrent in gliomas - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list