KYSE-520

ACC 371

Cell line:

KYSE-520

DSMZ no.:

ACC 371

Species:

human (Homo sapiens)

Cell type:

esophageal squamous cell carcinoma

Origin:

established from the moderately differentiated invasive eosphageal squamous cell carcinoma resected from lower intra-thoracic esophagus of a 58-year-old woman prior to treatment (tumor had invaded the adventitia); described as carrying TP53 mutation and amplification of the oncogene MYC

Reference(s):

14954, 14955, 15006, 14646, 18232

Biosafety level:

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

epitheloid cells growing adherently as monolayers, occasional giant cells present (ca. 1%); image; image; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 0.5-1.0 x 10⁶ cells/80 cm²; split confluent culture 1:6 to 1:8 every 3-4 days using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 30 hours

Harvest:

cell harvest of ca. 4-5 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human flat-moded hypotriploid karyotype with 8% polyploidy - 58-60<3n>XX, -X, +1, +1, -2, -2, -4, -5, -6, +7, -8, -10, -10, -11, -12, -12, -13, -14, -15, -16, -17, -18, +20, +20, -21, -21, -22, -2, +7-8mar, add(X)(p11), add(X)(q11), add(1)(q11), der(1)del(1)(q12)t(1;7)(p36;q21), der(1)del(1)(q12)t(1;5)(p36;q11), del(1)(q21), del(1)(p34), add(2)(q37), del(3)(q13), add(4)(p11), add(4)(p15), add(5)(q11)x1-2, add(6)(q1?), t(6;15)(p10;q10)x1-2, del(7)(q11)x1-2, add(7)(p22), add(9)(p23), der(11)add(11)(p15)t(11;12)(q24;q11), add(11)(p15), add(16)(q2?3), add(17)(q24), add(18)(p11), add(19)(p13)del(22)(q13)

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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