KYSE-180

human (Homo sapiens)

esophageal squamous cell carcinoma

established from the well differentiated squamous cell carcinoma resected from middle intra-thoracic esophagus of a 53-year-old man prior to treatment (tumor was invading contiguous structures)

14955, 15006, 14954, 14646, 18232

1

A

epitheloid cells growing as adherent monolayer; image; image; image

90-95% RPMI 1640 + 5-10% h.i. FBS

seed out at ca. 2-3 x 10⁶ cells/80 cm² in 10-15 ml; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA

at 37 °C with 5% CO₂

ca. 40-50 hours

cell harvest of ca. 30 x 10⁶ cells/175 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hyperdiploid karyotype with 11% polyploidy - 55(50-57)<2n>XX, +1, +1, +3, +7, +7, +8, -14, +16, -17, -17, -18, -18, +5-8mar, del(X)(q23), add(1)(q32), der(1;6)(q10;p10), der(1;13)(q10;q10), add(3)(p26), add(5)(q25), add(6)(p11), del(6)(q23), add(7)(p11), del(7)(p11), add(7)(q35), i(7q), del(8)(p22), add(10)(p11), add(11)(q11), del(11)(q11q14), add(13)(p11), add(13)(q33), add(14)(p11), add(15)(p11), add(21)(p11)

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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