SUP-B15
ACC 389
Cell line:
SUP-B15
DSMZ no.:
ACC 389
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the bone marrow of a 9-year-old boy with acute lymphoblastic leukemia (B cell precursor ALL) in second relapse in 1984; described in the literature to carry the ALL-variant (m-bcr) of the BCR-ABL1 fusion gene (e1-a2)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% McCoy's 5A + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; it is advisable to culture the cells initially in 24-well-plates, cell growth may be slow during the first week after thawing; maintain at ca. 0.5-2.0 x106 cells/ml; split 1:2 to 1:3 about once or twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml; maximum density of ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD19 +, CD34 +, CD37 -, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype - 46<2n>XY, der(1)t(1;1)(p11;q31), add(3)(q2?7), der(4)t(1;4)(p11;q35), t(9;22)(q34;q11), add(10)(q25), ?del(14)(q23q31), der(16)t(9;16)(q11;p13) - resembles published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list