VM-CUB-1
ACC 400
Cell line:
VM-CUB-1
DSMZ no.:
ACC 400
Species:
human (Homo sapiens)
Cell type:
urinary bladder transitional cell carcinoma
Origin:
established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described in the literature to carry a p53 point mutation
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30-45 hours
Harvest:
cell harvest of ca. 40 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(10)(p11), del(10)(q?22?24), add(12)(p11)x2, add(15)(p11), add(21)(p11) - sideline with i(5q)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list