CAL-78
ACC 449
Cell line:
CAL-78
DSMZ no.:
ACC 449
Species:
human (Homo sapiens)
Cell type:
chondrosarcoma
Origin:
established from the recurrence of a dedifferentiated chondrosarcoma (grade III) of the muscle of the thigh from a 76-year-old man in 1989; tumorigenic chondrosarcomatous differentiation of xenografted tumor
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent fibroblast-like cells growing as monolayer; the culture contains a large amount of cellular debris
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/25 cm2; cells grow extremely slowly; split confluent culture 1:2 every 10-12 days using trypsin with EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 240 hours
Harvest:
cell harvest of ca. 10-14 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list