ESS-1
ACC 461
Cell line:
ESS-1
DSMZ no.:
ACC 461
Species:
human (Homo sapiens)
Cell type:
endometrial stromal sarcoma
Origin:
established from an endometrial stromal sarcoma of the uterus of a 76-year-old woman; cells were described as demonstrating a mainly mesenchymal phenotype with signs of epithelial characteristics within foci and an extensively multilayered growth pattern
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 every week using trypsin/EDTA, but change the medium more frequently; maximum density of ca. 10-15 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 120 hours
Harvest:
cell harvest of ca.10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human karyotype with hyper-diploid/tetraploid and -octaploid clones present in approx. equal numbers - 47(45-47)<2n>XX, +19, der(3)t(3;6)(q2?8;p11-12), add(6)(q27), add(19)(p11) - sideline with i(2q) - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list