JEG-3
ACC 463
Cell line:
JEG-3
DSMZ no.:
ACC 463
Species:
human (Homo sapiens)
Cell type:
choriocarcinoma
Origin:
established from a human gestational choriocarcinoma which was taken at autopsy from a cerebral metastasis; cells were initially propagated in hamster cheek pouches by serial passage prior to cultivation in culture; serial sister cell line of cell line BEWO (DSMZ ACC 458)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Ham's F12 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; maintain at ca. 10 x 106 cells/175 cm2; split confluent culture up to 1:10 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 24 hours
Harvest:
cell harvest of ca. 25 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 12% polyploidy - 71(69-72)<3n>XXY, +2, +5, +9, -11, -13, -18, +4-6mar, ?del(X)(p11.4), der(4)t(4;11)(p16;q13), add(6)(q2?7)x1-2, add(7)(q32), add(7)(p22), i(13q), add(15)(p11), add(19)(p13) - sideline with add(8)(p1?), add(10)(q21), ?del(18)(q22) - resembles published karyotype and (marginally) that of BEWO
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list