Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 14-year-old boy with ALL and thymoma at diagnosis in 1973. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round to polymorph cells growing singly or in clusters in suspension;
image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; after thawing, we recommend to use initially 24-well-plates and 20% FBS; cell viability may be as low as 50% after thawing and cells may do not grow during the first week, then recover slowly; in this first week do not dilute, only exchange spent medium; when growing is resumed, split saturated culture 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density of about 4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
pseudodiploid human karyotype with 8% polyploidy - 46<2n>XY, der(1)t(1;16)(q22;p11-12)add(16)(p13), del(2)(p24), del(3)(p11), der(5)t(1;5)(q22-24;q32-33), r(16)(?p12?q12) - sideline with -3 instead of del(3) - resembles published karyotype
Molec. Genetics:
expression of TLX3 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
