MUTZ-5
ACC 490
Cell line:
MUTZ-5
DSMZ no.:
ACC 490
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established in 1998 from the peripheral blood of a 26-year-old man with B cell precursor acute lymphoblastic leukemia (BCP-ALL) at relapse
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
cells grow singly or in small clusters in suspension, irregular cell forms; image
Medium:
80% McCoy's 5A + 20% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 1 x 106 cells/ml; after thawing, culturing in 24-well-plate is of advantage; split saturated culture 1:2 to 1:3 every 2-4 days; minimum cell density of 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70-80 hours
Harvest:
maximum density at 3.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, CD71 +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 45<2n>X, -Y, t(12;13)(p12;q13-14)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list