MEC-2
ACC 500
Cell line:
MEC-2
DSMZ no.:
ACC 500
Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 1994 from the peripheral blood of a 62-year-old man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL) prior to therapy; serial sister cell line of MEC-1 (DSM ACC 497)
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Iscove's MDM + 10% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; after thawing, during the first week cells should be kept with 20% FBS; starting the culture in 24-well-plate is of advantage; viability may not exceed 80%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximal density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MEC-1 (ACC 497)
Cytogenetics:
human flat-moded hypertriploid karyotype with 7% polyploidy - 75-80<3n>XX/XXY, -6, -9, -10, +11, +12, -13, +15, -17, -21, +10mar - add(X)(p22)x2, inv(12)(?p12.2?q24.1)x2, add(18)(q2?1) - karyotypically distinct from MEC-1 (as reported)
Viruses:
PCR: EBV +, HBV -, HCV-1 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list