ONCO-DG-1 - Discontinued

human (Homo sapiens)

ovary adenocarcinoma (cell line OVCAR-3)

Caution: As determined by STR analysis, ONCO-DG-1 is genetically identical to the human ovary adenocarcinoma cell line OVCAR-3. ONCO-DG-1 must not used as a model for thyroid carcinoma.

1

A

epithelial-like adherent cells with large vacuoles growing as monolayers; image; image

90% RPMI 1640 + 10% h.i. FBS

seed out at ca. 1 x 10⁶ cells/80 cm²; split confluent culture 1:5 every 3-4 days using trypsin/EDTA (for 10 min)

at 37 °C with 5% CO₂

ca. 30 hours

cell harvest of about 10 x 10⁶ cells/175 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

initial contamination was eliminated with ciprofloxacin (Ciprobay), then negative in DAPI, microbiological culture, and continuously in PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in the STR profile of cell line OVCAR-3.

human hypotriploid karyotype with 8% polyploidy - 59(56-61)<3n>XX, -X, +1, -2, -4, -6, -8, -9, -13, -13, -14, -15, -15, -17, -17, -18, -19, -21, -22, +7-9mar, +0-3 dm, add(1)(p3?5), add(1)(p2?1), add(1)(q11), der(3;15)(p10;q10), add(3)(p14), del(5)(q23), add(6)(q23), der(7;14)(q10;q10), del(9)(p23), add(10)(p11), add(11)(p11)x2, del(?11)(q13q14.2), der(12;18)(q10;q10), add(13)(p11), add(14)(p11), add(16)(q23-24), add(17)(p11), add(21)(p11)x1-2 - carries large submetacentric hsr marker

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

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