PL-21
ACC 536
Cell line:
PL-21
DSMZ no.:
ACC 536
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 24-year-old man with refractory acute promyelocytic leukemia after mediastinal granulocytic sarcoma; however, PL-21 cells do not carry the t(15;17); based on morphology, surface marker expression of CD14/CD68 and expression of the monocyte-specific esterase, PL-21 cells appear to be rather of monocytic origin; differentiation is inducible by phorpbol ester; cells carry an FLT3 internal tandem duplication
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 1.0-1.5 x 106 cells/ml; minimum density at ca. 0.5 x 106 cells/ml; maximum density at ca. 2 x 106 cells/ml; cultivate cells in a 12- or 24-well-plate after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 (+), CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertetraploid karyotype - 93(83-96)<4n>XXYY, +3, +5, +5, +6, +6, +7, -9, -10, -11, -12, +13, -14, -17, -21, +22, +22, +mar, del(1)(p11), i(5p)x2-3, der(6)t(6;15)(p25;q24), der(13)ins(13;13)q32;q?q?)x3-4 - no cytogenetic evidence of PML-RARA fusion - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list