DB
ACC 539
Cell line:
DB
DSMZ no.:
ACC 539
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from ascites of a 45-year-old man with diffuse large cell lymphoma; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; minimum density at ca. 0.4 x 106 cells/ml; after thawing cultivate in 6-well-plates during the first 2 weeks
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 -, CD10 +, CD13 -, CD20 +, CD37 +, HLA-DR -, cyIgG +, cy IgM -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype - 80(79-81)<3n>XXXYY, -3, +7, +7, +8, -9, +11, -13, +16, +18, +20, +20, +20, +21, +22, +mar, der(4)t(X;4)(q11;p13), add(5)(q11), t(10;20)(q24;p11), ?inv(13)(q21.2q32), t(14;18)(q32;q21), del(16)(p13.2), der(18)(t(14;18)(q32;q21) - carries t(14;18) associated with B-cell lymphoma
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list