Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 5-year-old girl at relapse of acute lymphoblastic leukemia (ALL) in 1990; reported to carry the t(4;11) KMT2A-AFF1 (MLL-AFF1; MLL-AF4). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Morphology:
round to polygonal single rather small cells in suspension;
image;
image;
image
Medium:
90% Iscove's MDM + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days; maximum density at ca. 3-4 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 3-4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD4-, CD11b-, CD13+, CD14-, CD15+, CD19+, CD33+, CD34-, cyCD68-, HLA-DR+, sm/cyIgG-, sm/cyIgM-, sm/cykappa-, sm/cylamda-;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 1.5% polyploidy - 45(40-46)<2n>XX, -13, t(4;11)(q21;q23), del(7)(p14) - carries t(4;11) with breakpoints at AFF1 (AF4) and KMT2A (MLL) - matches published karyotype
Molec. Genetics:
expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
