EN
ACC 564
Cell line:
EN
DSMZ no.:
ACC 564
Species:
human (Homo sapiens)
Cell type:
endometrial carcinoma
Origin:
established from the primary endometrial (cervical) carcinoma (stage IIIC) which was histologicall poorly differentiated from a 50-year-old woman prior to therapy
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out 1.5 x 106 cells in 80 cm2; maintain at 0.1-0.2 x 105 cells/cm2; after recovery from N2 cell growth is slow (doubling time > 120 h)
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50-80 hours
Harvest:
cell harvest of ca. 1 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-tetraploid karyotype with 3% polyploidy - 79-97<4n>XXX/XXXX, -13, +2mar, 0-4 dm, der(1;7)(q10;q10)x2 - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list