SU-DHL-10
ACC 576
Cell line:
SU-DHL-10
DSMZ no.:
ACC 576
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the pleural effusion of a 25-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse histiocytic lymphoma; cell line carries EZH2 Y641F mutation (own results); assigned to GCB-like lymphoma subtype (germinal center B-cell)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round lymphoid cells growing singly or in clumps in suspension; image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.3-0.8 x 106 cells/ml in 12-well-plates; after thawing viability may be low, but cells will soon start to grow vigorously; split saturated culture up to 1:10 every 2-4 days; maintain at 0.3-1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 15-30 hours
Harvest:
cell harvest of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, HLA-DR +, cyIgG +, cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype - 47(43-48)<2n>XY, +7, der(8)t(X;8)(q25;p23)t(8;X)(q24;q26)t(X;14)(q28;q32), del(10)(q22q24), der(11)t(Y;11)(q11;q25), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - carries concurrent rearrangements of IGH with MYC and BCL2 - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list