KMS-12-PE

human (Homo sapiens)

multiple myeloma

established in 1987 from the pleural effusion of a 64-year-old woman with refractory, terminal multiple myeloma (Ig-non-producing) after combination chemotherapy; carries t(11:14)(q13:q32) rearrangement; sister cell line of KMS-12-BM (DSMZ ACC 551)

14799, 14817, 14818

1

A

small round cells growing singly or occasionally in small clumps in suspension; image

80% RPMI 1640 + 20% h.i. FBS

seed out at ca. 0.5 x 10⁶ cells/ml; cells may be cultured initially in 24-well plates; split ratio of 1:2 to 1:4 every 2-5 days; maintain at 0.5-1.5 x 10⁶ cells/ml

at 37 °C with 5% CO₂

ca. 60-80 hours

maximum density at ca. 2.5 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD4 -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD34 -, CD38 +, CD138 +, HLA-DR +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hypodiploid karyotype with 8% polyploidy; 42(40-47)<2n>X,-X, +1, +7, -14, +16, +18, -22, der(1;16)(q10;p10), der(1)(1pter->q12::18q1?-q2?1::14q21.2->q32.32::11q.13->qter)qdp(1q12.1->q12::18q1?->q21), der(3)dup(3)(q26q29)t(3;21)(q29;q11.2), del(4)(q26), der(5)t(5;17)(p10;q10), der(6)t(1;6)(q32;q22), der(7)t(7;10)(p21;p13), der(8)ins(8;14)(q24;q32.3q32.3)x2, ider(9)(q10)t(9;11)(q24;q11), der(10;16)(q10;q10), der(11)t(11;14)(q13;q32), der(11)t(9;11)(q13;q34), del(13)(q11), add(16)(p11), der(16)t(13;16)(q31;q21) der(17)t(1;17)(?;p13), add(18)(q21)x2, del(19)(q11); carries simultaneous IGH-CCND1 and IGH-MYC rearrangements; resembles published karyotype

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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