Species:
human (Homo sapiens)
Cell type:
acute myelocytic leukemia
Origin:
established in 1984 from the leukemic cells of a 56-year-old patient with erythroleukemia (AML-M6), representing the end stage of a previously identified myelodysplastic syndrome; described in the literature to differentiate along the erythroid or megakaryocytic/monocytic lineage after stimulation with erythroid inducers or phorbol esters, respectively; described in the literature to constitutively and inducibly secrete platelet-derived growth factor (PDGF)-like proteins; contamination with Mycobacterium was eliminated with kanamycin. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round cells growing singly or in clusters in suspension, occasionally a few cells become adherent with elongated morphology;
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Medium:
80% Iscove's MDM + 20% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at 1-1.5 x 106 cells/ml; optimal split ratio 1:3 to 1:5 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36 hours
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD14 -, CD19 -, CD33 (+), CD71 +;
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy; 51(46-51)<2n>XX, +6, +8, +20, -21, +3mar, der(X)t(X;8)(q23;q23), add(9)(p23)del(9)(p12p21), t(10;12)(p12;p12), del(17)(q11q21.1); no reference karyotype available
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
