GI-ME-N
ACC 654
Cell line:
GI-ME-N
DSMZ no.:
ACC 654
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
established in 1986 from a bone marrow metastasis of a 42-month-old girl with stage IV neuroblastoma (supposedly N-type) after 6 months of chemotherapy; described to carry NF1 gene mutations
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 every 2-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list