Leibniz Institute DSMZ: Details

Cell line:

CAKI-1

DSMZ no.:

ACC 731

Species:

human (Homo sapiens)

Cell type:

kidney carcinoma

Origin:

established in 1971 from the cutaneous metastasis of the kidney adenocarcinoma of a 49-year-old man

Reference(s):

Biosafety level:

1

Permissions and restrictions:

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

epithelioid cells growing adherently in monolayers; image; image

Medium:

80% McCoy´s 5a + 20% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/75 cm² flask; split culture 1:2 to 1:4 every 3-5 days using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 40-60 hours

Harvest:

cell harvest of ca. 8-14 x 10⁶ cells/175 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €