I83-LCL
ACC 764
Cell line:
I83-LCL
DSMZ no.:
ACC 764
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells (from patient with chronic B cell leukemia)
Origin:
normal B-cell derived lymphoblastoid cell line established in 1994 from a 71-year-old man with chronic lymphocytic leukemia (CLL); autologous to cell line I83-E95 (DSM ACC 763)
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing mainly in clumps in suspension
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.4-1 x 106 cells/ml; optimal split ratio 1:2 - 1:4 every 2-4 days; split culture or change medium once or twice a week to eliminate a lot of cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximum density at ca. 1.3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; in the referenced paper, this cell line was described as IgM +, lambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype; 83-92<4n>XXYY, -2, -6, +10, +10, -16, der(10)t(2;10)(q21.2;q21)
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list