U-2904
ACC 768
Cell line:
U-2904
DSMZ no.:
ACC 768
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1991 from the pleural effusion of a 59-year-old man with centroblastic-centrocytic B-cell non-Hodgkin-lymphoma at diagnosis that secondarily transformed to centroblastic lymphoma after radiotherapy; cells were harvested after chemotherapy; described in the literature to carry t(8;14) and t(14;18) affecting MYC-IGH and IGH-BCL2, respectively
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.7 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every second day; maintain at ca. 0.5-2.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 - 35 hours
Harvest:
maximum density at ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-diploid karyotype;
43-47<2n>X-Y, +4, -13, +20, der(1)t(1;22)(p11;q11), inv(1)(q11q21), del(6)(q11), t(8;14)(q24;q32), der(10)t(10;13)(p13;q13), t(14;18)(q32;q21), del(16)(q22); carries biallelic rearrangements of IGH with MYC and BCL2, respectively; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list