L4.5
ACC 772
Cell line:
L4.5
DSMZ no.:
ACC 772
Species:
murine (mouse) (Mus musculus)
Cell type:
derivative of L-929
Origin:
derived from L-929 cells by transfection with vector pTEJ-8 (mediating geneticin-resistance) containing the cDNA for murine CD40L in 1996; cells are described in the literature to express murine CD154
Biosafety level:
1, GMO-S1
Risk assessment:
The cell line L4.5 derived from L-929 cells by transfection with the geneticin-resistance mediating vector pTEJ-8 containing the cDNA for murine CD40L and thus has to be regarded as genetically modified organism (GMO). Neither the parental cell line nor the plasmid and its expression products are harmful to man. Hence, the cell line is categorized risk group 1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
fibroblastoid cells growing as monolayer
Medium:
90% Iscove's MDM + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:10 - 1:20 once or twice a week using trypsin with EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list