PG-EBV
ACC 776
Cell line:
PG-EBV
DSMZ no.:
ACC 776
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells
Origin:
normal B-cell derived lymphoblastoid cell line established in 1988 from a man with chronic lymphocytic leukemia (CLL) by immortalization with B95-8 derived EBV; autologous to cell line PGA-1 (DSM ACC 766); cell line is also known as PG/B95-8
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml in a small culture flask; split 1:2 - 1:3 twice a week; maintain at ca. 0.5 - 1.2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
maximum density at ca. 1-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype; 46(43-46)<2n>XY, der(Y)t(Y;2)(qter;p14); sdl with -2; related to published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list