MOLM-14
ACC 777
Cell line:
MOLM-14
DSMZ no.:
ACC 777
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndrome (MDS, refractory anemia with excess of blasts, RAEB); carries internal tandem duplication of FLT3; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-13 (ACC 554)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml in 24-well-plates; maintain at ca 0.5-1.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MOLM-13
Cytogenetics:
human hyperdiploid karyotype; -49(46-50)<2n>XY, +6, +8, +13, der(2)t(1;2)(q31;q35), ins(11;9)(q23;p22p23), del(14(q23q32.3), del(16)(q11.2q13.1); sdl with der(2) but with tetrasomy 8 and/or i(8)(q10); carries insertional variant of t(9;11) recurrent in AML, effecting rearrangement of KMT2A (MLL) with MLLT3 (AF9); ins(11;9) plus trisomies 8 and 13 also present in sister cell line MOLM-13 which it closely resembles
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list