HC-11
ACC 780
Cell line:
HC-11
DSMZ no.:
ACC 780
Species:
murine (mouse) (Mus musculus)
Cell type:
normal mammary epithelial
Origin:
derived in 1987 as a prolactin-responsive subclone of the COMMA-1D cell line which was established from normal mammary epithelial cells of an adult female BALB/c mouse
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epithelioid adherent cells growing as monolayer
Medium:
90% RPMI 1640 + 10% h.i. FBS + 5 µg/ml insulin (bovine or human) + 10 ng/ml EGF + 4 mM L-glutamine
Subculture:
seed out at ca. 0.3-1 x 106 cells/25 cm2 flask; split confluent culture 1:2 - 1:4 every 2-4 days using trypsin/EDTA for about 10 min
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-80 hours
Harvest:
cell harvest of ca. 6 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list