JIH-5
ACC 788
Cell line:
JIH-5
DSMZ no.:
ACC 788
Species:
human (Homo sapiens)
Cell type:
acute biphenotypic leukemia
Origin:
established in 2009 from leukemic bone marrow cells of a 21-year old woman with mixed-phenotype acute leukemia (MPAL) at second relapse; cells express typical antigens of myeloid as well as B-lymphoid lineages; cells were described in the literature to express EP300-ZNF384 fusion gene
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% Iscove's MDM + 20% h.i. FBS
Subculture:
seed out at ca. 1.5 x 106 cells/well in 24 well plates, subsequently cells may be grown in flasks, optimal split ratio 1:1.5 - 1:2 every 5-7 days, maintain between 1 and 2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 5-7 days
Harvest:
maximum density at ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 +, CD15 +, CD19 +, CD20 -, CD21 -, CD34 -, CD37 -, CD38 +, CD138 -, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
expression of fusion genes EP300-ZNF384 and CHD4-EP300 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list