170-MG-BA
ACC 804
Cell line:
170-MG-BA
DSMZ no.:
ACC 804
Species:
human (Homo sapiens)
Cell type:
glioblastoma
Origin:
etablished in 2015 from the temporal lobe brain tumor diagnosed as glioblastoma multiforme of a 74-year-old woman
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
spindle-like epithelioid cells growing adherently in monolayers
Medium:
90% MEM (with Earl´s salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids
Subculture:
seed out at ca. 0.5-1 x 106 cells/175 cm2 flask; split 1:3 to 1:6 every 3-7 days using trypsin/EDTA
Incubation:
at 37 °C with CO2
Doubling time:
ca. 40-60 hours
Harvest:
cell harvest of ca. 5-11 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
Highly rearranged human karyotype with focal genomic amplification of EGFR in two der(17) marker chromosomes
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list