MC-116
ACC 82
Cell line:
MC-116
DSMZ no.:
ACC 82
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the pleural effusion of a patient with B cell lymphoma (undifferentiated lymphoma); cells were described in the literature to be EBV-negative, to carry the t(8;14) and to express mRNA of the proto-oncogene BCL2
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-85% RPMI 1640 + 15-20% h.i. FBS
Subculture:
maintain at 1-2 x 106 cells/ml; split ratio of 1:2 to 1:3; seed out at ca. 1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 24-48 hours
Harvest:
maximal density at 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda +
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype with 1.5% polyploidy - 46(37-46)<2n>XY, dup(1)(q21q32), t(8;14)(q24;q32), del(10)(q23)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list