JVE-222
ACC 821
Cell line:
JVE-222
DSMZ no.:
ACC 821
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from the primary tissue of an ascending colorectal adenocarcinoma (Dukes stage B2) of a 60-year-old woman; cells were described in literature to carry mutations in the KRAS, PIK3CA, and APC genes, a partial methylation of the CDKN2A promoter and to display microsatellite instability (MSI-H)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently in islets and monolayers; culture may contain cell debris
Medium:
90% mixture of Ham's F12 + DMEM (1:1) + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/25 cm2;
split conflent culture 1:2 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
cell harvest of 25-30 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list