Leibniz Institute DSMZ: Details

Cell line:

MCA-B1

DSMZ no.:

ACC 828

Species:

canine (dog) (Canis lupus familiaris)

Cell type:

acanthomatous epulis

Origin:

odontogenic tumor of the gingiva (acanthomatous epulis); established in 1992 from the tumor tissue from a 10-year old domestic dog (Akita bred, Japan)

Reference(s):

27645, 27648

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

epithelioid cells growing adherently in monolayers with pavement-like colonies; image;image; image

Medium:

mixture of Ham's F12 + DMEM (1:1) + 5-10% h.i. FBS

Subculture:

seed out 1-2 x 10⁶ cells/80 cm²; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 24 hours

Harvest:

cell harvest of 6-8 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Species validation:

Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Canis lupus familiaris species.

Viruses:

PCR: MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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