U2OS#18-RAD51D-4/D+
ACC 840
Cell line:
U2OS#18-RAD51D-4/D+
DSMZ no.:
ACC 840
Species:
human (Homo sapiens)
Cell type:
osteosarcoma
Origin:
Genetical modification of U2OS#18 (DSMZ ACC 832), a reporter cell line for studying sister chromatid recombination, SCR). This cell line belongs to the series of genetically disrupted RAD51 paralogs with reversion of the disruption by retroviral transfection with the functional gene (the non-complemented sister cell line is ACC 835). Cells are described in the literature to be puromycin and hygromycin resistant
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to verify antibiotic resistance, 1 µg/ml puromycin and/or 150 µg/ml hygromycin should be added)
Subculture:
seed out at 1 x 106 cells/175 cm2;
split confluent culture 1:4 to 1:10 every 4-10 days using trypsin/EDTA; after thawing, cells grow rather slowly (doubling time about 80 h)
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 20-50 hours
Harvest:
cell harvest of 8-10 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
frameshift mutations in exon 1 of RAD51D were verified by sequence analysis and forced expression of RAD51D in the retrovirally transfected cell line was confirmed by RQ-PCR analysis
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list