NT-27a
ACC 857
Cell line:
NT-27a
DSMZ no.:
ACC 857
Species:
rat (Rattus norvegicus)
Cell type:
hepatocellular tumor
Origin:
subclone of NT-27 which was established ca. 1986 from the liver of a male Wistar AF/Han rat treated with N-methyl-N-nitrosourea
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently in clusters as monolayer
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:5 to 1:7 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24-30 hours
Harvest:
cell harvest of ca. 25 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Viruses:
PCR: MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list