RC-K8
ACC 561
Cell line:
RC-K8
DSMZ no.:
ACC 561
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the peritoneal effusion of a patient with lymphoma, described at the time as true histiocytic lymphoma, in 1984 (terminal, refractory stage); assigned to GCB-like lymphoma subtype (germinal center B-cell); cells carry a BCL6 translocation
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round to polymorph cells growing mainly in suspension or in small clusters; image
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at 0.8 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 4% polyploidy - 47(42-49)<2n>XY, -13, -15, +2 mar, der(Y)t(Y;5)(qter;?p15), der(?1)t(1;13)(p32;q11)del(1)(q11), del(2)(p11p13), dup(2)(p13p16), add(3)(q2?), der(5)?inv(5)(q15q32)add(5)(q32), inv(5)(p?13q?32), add(7)(q31), der(8)(t(8;8)(p12;q11), del(9)(p24)x2, der(10)t(9;10)(?p24;p11), t(11;14)(q23;q32), der(14)t(X;14)(p11;p11), der(15)(t(X;15)(p11.3;p11), add(17)(p11), der(20)t(13;20)(q11;q13) - carries breakpoints at REL and IGH-DDX6 - matches published karyotype
Molec. Genetics:
BCL6 translocation was verified by genomic PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list