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CharacterizationCharakterisierung

Characterization


Beside the considerable tests for quality and identity control we routinely perform further investigations on the characteristics of the cell lines from the public repository. These include the determination and/or the verification of a described tissue type of a cell line by tissue-specific antibodies. Furthermore, we describe the karyotype and verify the expression of aberrantly expressed genes, e.g. fusion genes, genes with characteristic point mutations, amplifications etc. All these investigations contribute to our aim to elaborate individual characteristics which, of course, can also serve as authetication parameters of a given cell line. However, we are in no position to verify and further examine every possible aspect of a given cell line. Some basic characteristics of most cell lines have been described in the literature quoted. Additional papers have been published on each cell line that should be consulted to obtain a general overview of a cell line.

Certain characteristics claimed to be expressed by a cell line might not be found reproducibly on every subclone or even the seeding culture. We cannot be held responsible for claims made by other scientists in the literature regarding certain features of the cell lines that might not be identified on the cell culture we provide.

 

Cytogenetics.

Cytogenetic data are of great value for cell line authentication. Most cell lines display unique karyotypes and thus provide reference data for the identification of human tumor cell lines. Fluorescence in situ hybridization is currently the standard method for the analysis of entire chromosomes, segements thereof, or single genes. More

Immunophenotyping.

Marker proteins have proven to be helpful in the diagnosis of solid tumors when conventional cytology alone does not provide a clear result. Most human cell lines were derived from tumors. Therefore, we routinely test the expression of tissue markers on all human cell lines using a panel of well-characterized monoclonal antibodies (mAbs) to confirm their tissue origin. More

Cancer-type specific mutations.

 

Most cell lines of the public collection derive from tumor cells. In many cases the tumors exhibit type-specific mutations which contribute to tumorigenesis and are, therefore, relevant for diagnostic studies and research. These genetic markers may be identified by specific PCR assays, supporting informing characterization of the cell lines and their authentication. More