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Frequently Asked Questions (FAQ)

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Questions and answers (Phages)

fold faq Why is there a general price for all phages, independent of the form of delivery?

Propagation and preservation procedures for phages are different compared to bacteria. The DSMZ leaves it to the customer to choose between two delivery forms: phages in liquid or dried in ampoules.

fold faq How do I work with phages received from DSMZ, where can I find methods?

Phage methods can be found under “Supply, storage, propagation and purification of phages”.

fold faq What do phages always need for adsorption to the host bacterium?

Addition of 5 mM MgSO4 or CaCl2 is strongly recommended. Avoid fast shaking or strong agitation of phages in liquids.

fold faq Does an order on a phage include its host, are phage and host delivered together?

No, phage and host have to be mentioned separately on the order, they are different order positions. Please, use only the host strain recommended by the DSMZ.

fold faq In what form will the phage be delivered, dried or liquid?

The form of delivery is the customer´s choice: phages are available as suspensions delivered at ambient temperature (1 mL) or as vacuum-dried samples on filter paper in glass ampoules. The form of delivery is independent of the destination. Few phages are available in one form only.

fold faq How do I know the titre of a phage suspension supplied by DSMZ?

Phage suspensions supplied by DSMZ have checked and documented sufficient phage activity to be used for further propagation.

fold faq Can phage suspensions be stored in the refrigerator?

Most phages store well in the refrigerator unfrozen for some weeks or longer but DSMZ generally recommends immediate use, see Terms and Conditions. Do not freeze phages without adding a cryoprotectant, e. g. 10% glycerol.

fold faq Does a phage plate only on the host strain recommended by DSMZ?

Host spectra of phages are not routinely tested by DSMZ so that successful lysis is only guaranteed for the phage/host system used and approved by DSMZ.

fold faq What is the difference between dried bacterial strains and filter-dried phages?

Bacterial cell suspensions are dried together with a protectant and a pellet forms that can be rehydrated, phages are dried as suspensions on filter paper strips.

fold faq Does the DSMZ “Opening of Ampoules and Rehydration of Dried Cultures” scheme apply to the revitalisation of dried phages supplied in glass ampoules?

No, see procedure given on our homepage: “Revitalisation of Vacuum-Dried Phages”.

fold faq Are phages allocated to risk groups?

Phages are viruses that infect bacteria only, they do not infect humans, animals or plants and are therefore officially allocated to the risk group 1.

fold faq Does the DSMZ provide isolated phage DNA?

No, currently the DSMZ exclusively offers phages in their complete natural form.

fold faq Does the DSMZ provide genome sequence data of phages?

No, the DSMZ recommends to search at NCBI or www.phantome.org

fold faq Does the DSMZ offer a phage identification service?

No, this service is not offered.