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DetailsCatalogue
Cell line:
K-562
DSMZ no.:
ACC 10
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the pleural effusion of a 53-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1970; cells carry the BCR-ABL1 e14-a2 (b3-a2) fusion gene; this subclone (ACC 10) weakly expresses MHC class I (image); other subclones (ACC 86 and ACC 92) are MHC class I negative (image) and might be used in in-vitro natural killer assays; cells produce hemoglobin
Reference(s):
14479, 14719, 14720, 15215, 15216
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
round large, single cells in suspension; image; image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-1.0 x 106 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximal density at 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays
Immunology:
CD3 -, CD14 -, CD15 +, CD19 -, CD33 +, CD71 +, CD235a +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH, NP
Cytogenetics:
human hypotriploid karyotype without sharp mode - 61-68<3n>XX, -X, -3, +7, -13, -18, +3mar, del(9)(p11/13), der(14)t(14;?)(p11;?), der(17)t(17;?)(p11/13;?), der(?18)t(15;?18)(q21;?q12), del(X)(p22) - two markers appear from FISH to have arisen from Ph
Molec. Genetics:
expression of fusion gene BCR-ABL1 (e14-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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