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DetailsCatalogue
Cell line:
MCF-7
DSMZ no.:
ACC 115
Species:
human (Homo sapiens)
Cell type:
breast adenocarcinoma
Origin:
established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma (after radio- and hormone therapy) in 1970; cells were described of being positive for cytoplasmic estrogen receptors and having the capability to form domes
Reference(s):
14975
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing as monolayers ; image
Medium:
90% RPMI 1640 + 10% h.i. FBS + MEM non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin
Subculture:
split confluent culture 1:2 to 1:4 every 2-6 days using trypsin/EDTA; seed out at ca. 0.1-0.2 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours (range 30-72 hours)
Harvest:
about 50-60 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human hypotetraploid karyotype with 8% polyploidy - 86(80-87)<4n>XX, -X, -X, -1, -1, +6, +7, -9, -10, -10, -12, -13, -13, +14, -17, -17, -18, -18, -20, -20, -20, -22, +6-8mar, add(X)(q27), add(2)(q35-37)x1-2, add(5)(p15), del(6)(q25)x1-2, add(7)(p11), add(7)(p14;hsr), add(10)(q23), del(11)(q21q23), add(15)(p11), i(15q), add(16)(q24), add(19)(q13), add(19)(p13)x1-2 - corresponds to published karyotype
Molec. Genetics:
expression of fusion gene BCAS4-BCAS3 confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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