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DetailsCatalogue
Cell line:
EM-2
DSMZ no.:
ACC 135
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome positive chronic myeloid leukemia (CML) in second relapse in 1980 (after bone marrow transplantation day +28); sister cell line of EM-3 (DSM ACC 134)
Reference(s):
14488, 14656, 14883, 15215
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
single, round to oval cells in suspension; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.2-1.0 x 106 cells/ml; split ratio of 1:2 every 2-3 days; seed out at ca. 0.3-0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 h
Harvest:
maximum density at about 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image
Fingerprint:
same DNA profile as EM-3 using multiplex PCR of minisatellite markers
Species:
confirmed as human with IEF of AST, MDH, NP
Cytogenetics:
human hypertriploid karyotype with hypotetraploid sideline - 74(70-86)<3n>X, -X, -X, +3, +4, +6, +6, +6, +8, -9, +11, -14, -14, +15, +17, -19, +21, +22, +mar - der(5)t(5;?)(q13-15;?), der(9)t(9;22)(q34;q11), i(17q)x2 - in some cells up to three copies of der(9) are present - the karyotype overlaps that of EM-3 (DSM ACC 134) and corresponds with those reported for both EM-2 and EM-3
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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