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DetailsCatalogue
Cell line:
NEURO-2A
DSMZ no.:
ACC 148
Species:
murine (mouse) (Mus musculus)
Cell type:
neuroblastoma
Origin:
established from the spontaneous tumor of a strain A albino mouse
Reference(s):
14667, 14826
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells; image; image ; image
Medium:
90% Dulbecco's MEM + 10% h.i. FBS + 1x non-essential amino acids
Subculture:
split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species:
Mus musculus origin confirmed by COI DNA barcoding
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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